Laboratory of Epigenetics and Genomic Integrity

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Laboratory of Epigenetics and Genomic Integrity

Jessica Tyler, PhD, Principal Investigator

Dr. Tyler’s laboratory focuses on discovering the mechanistic basis of the epigenetic regulation of aging, genomic integrity and gene expression. Our studies use a combination of molecular genetics in budding yeast, tissue culture studies, biochemistry and next generation sequencing approaches. The proteins and processes that we study are so fundamentally conserved through eukaryotic evolution, that we can take advantage of the strengths of the highly genetically malleable yeast system to guide our studies in human cells. In addition to learning how chromatin regulates fundamental processes in the cell, our studies are helping us to understand how defects in the chromatin structure lead to gene dysfunction and genomic instability, in turn causing aging and disease states including cancer.

Tyler Lab Image

Active Projects

  • Role of chromatin structure in maintaining genomic stability
  • Attaining longevity via chromatin dynamics
  • Discovering the mechanism of chromatin assembly

Active Grants

  • Chromatin’s Role in the Repair of Radiation-induced Damage
    PI: Jessica Tyler, PhD
    National Cancer Institute (NIH)

Learn more

Body

Laboratory of Epigenetics and Genomic Integrity

Jessica Tyler, PhD, Principal Investigator

Dr. Tyler’s laboratory focuses on discovering the mechanistic basis of the epigenetic regulation of aging, genomic integrity and gene expression. Our studies use a combination of molecular genetics in budding yeast, tissue culture studies, biochemistry and next generation sequencing approaches. The proteins and processes that we study are so fundamentally conserved through eukaryotic evolution, that we can take advantage of the strengths of the highly genetically malleable yeast system to guide our studies in human cells. In addition to learning how chromatin regulates fundamental processes in the cell, our studies are helping us to understand how defects in the chromatin structure lead to gene dysfunction and genomic instability, in turn causing aging and disease states including cancer.

Tyler Lab Image

Active Projects

  • Role of chromatin structure in maintaining genomic stability
  • Attaining longevity via chromatin dynamics
  • Discovering the mechanism of chromatin assembly

Active Grants

  • Chromatin’s Role in the Repair of Radiation-induced Damage
    PI: Jessica Tyler, PhD
    National Cancer Institute (NIH)

Learn more

Selected Publications EXPAND ALL ON THIS PAGE

The BRCA1-interacting protein Abraxas is required for genomic stability and tumor suppression

August 7, 2014
Castillo A, Paul A, Sun B, Huang TH, Wang Y, Yazinski SA, Tyler J, Li L, You MJ, Zou L, Yao J, Wang B.

The BRCA1-interacting protein Abraxas is required for genomic stability and tumor suppression

Cell Rep. 2014 Aug 7;8(3):807-17.

Castillo A, Paul A, Sun B, Huang TH, Wang Y, Yazinski SA, Tyler J, Li L, You MJ, Zou L, Yao J, Wang B.

PMID: 25066119; PMCID: PMC4149256.

Acetylated lysine 56 on histone H3 drives chromatin assembly after repair and signals for the completion of repair

July 25, 2014
Chen CC, Carson JJ, Feser J, Tamburini B, Zabaronick S, Linger J, Tyler JK.

Acetylated lysine 56 on histone H3 drives chromatin assembly after repair and signals for the completion of repair

Cell. 2008 Jul 25;134(2):231-43.

Chen CC, Carson JJ, Feser J, Tamburini B, Zabaronick S, Linger J, Tyler JK.

PMID: 18662539; PMCID: PMC2610811

MOF phosphorylation by ATM regulates 53BP1-mediated double-strand break repair pathway choice

July 10, 2014
Gupta A, Hunt CR, Hegde ML, Chakraborty S, Chakraborty S, Udayakumar D, Horikoshi N, Singh M, Ramnarain DB, Hittelman WN, Namjoshi S, Asaithamby A, Hazra TK, Ludwig T, Pandita RK, Tyler JK, Pandita TK.

MOF phosphorylation by ATM regulates 53BP1-mediated double-strand break repair pathway choice

Cell Rep. 2014 Jul 10;8(1):177-89.

Gupta A, Hunt CR, Hegde ML, Chakraborty S, Chakraborty S, Udayakumar D, Horikoshi N, Singh M, Ramnarain DB, Hittelman WN, Namjoshi S, Asaithamby A, Hazra TK, Ludwig T, Pandita RK, Tyler JK, Pandita TK.

Erratum in: Cell Rep. 2014 Jul 10;8(1):319.

PMID: 24953651; PMCID: PMC4300955.

The conformational flexibility of the C-terminus of histone H4 promotes histone octamer and nucleosome stability and yeast viability

April 27, 2014
Chavez MS, Scorgie JK, Dennehey BK, Noone S, Tyler JK, Churchill ME.

The conformational flexibility of the C-terminus of histone H4 promotes histone octamer and nucleosome stability and yeast viability

Epigenetics Chromatin. 2012 Apr 27;5(1):5.

Chavez MS, Scorgie JK, Dennehey BK, Noone S, Tyler JK, Churchill ME.

PMID: 22541333; PMCID: PMC3439350

Binding of the histone chaperone ASF1 to the CBP bromodomain promotes histone acetylation

March 25, 2014
Das C, Roy S, Namjoshi S, Malarkey CS, Jones DN, Kutateladze TG, Churchill ME, Tyler JK.

Binding of the histone chaperone ASF1 to the CBP bromodomain promotes histone acetylation

Proc Natl Acad Sci U S A. 2014 Mar 25;111(12):E1072-81.

Das C, Roy S, Namjoshi S, Malarkey CS, Jones DN, Kutateladze TG, Churchill ME, Tyler JK.

PMID: 24616510; PMCID: PMC3970516

Nucleosome loss leads to global transcriptional up-regulation and genomic instability during yeast aging

February 15, 2014
Hu Z, Chen K, Xia Z, Chavez M, Pal S, Seol JH, Chen CC, Li W, Tyler JK.

Nucleosome loss leads to global transcriptional up-regulation and genomic instability during yeast aging

Genes Dev. 2014 Feb 15;28(4):396-408.

Hu Z, Chen K, Xia Z, Chavez M, Pal S, Seol JH, Chen CC, Li W, Tyler JK.

PMID: 24532716; PMCID: PMC3937517

Mitotic phosphorylation of histone H3 threonine 80

January 1, 2014
Hammond SL, Byrum SD, Namjoshi S, Graves HK, Dennehey BK, Tackett AJ, Tyler JK.

Mitotic phosphorylation of histone H3 threonine 80

Cell Cycle. 2014;13(3):440-52.

Hammond SL, Byrum SD, Namjoshi S, Graves HK, Dennehey BK, Tackett AJ, Tyler JK.

PMID: 24275038; PMCID: PMC3956540

DANPOS: dynamic analysis of nucleosome position and occupancy by sequencing

February 1, 2013
Chen K, Xi Y, Pan X, Li Z, Kaestner K, Tyler J, Dent S, He X, Li W.

DANPOS: dynamic analysis of nucleosome position and occupancy by sequencing

Genome Res. 2013 Feb;23(2):341-51.

Chen K, Xi Y, Pan X, Li Z, Kaestner K, Tyler J, Dent S, He X, Li W.

PMID: 23193179; PMCID: PMC3561875

The C terminus of the histone chaperone Asf1 cross-links to histone H3 in yeast and promotes interaction with histones H3 and H4

February 1, 2013
Dennehey BK, Noone S, Liu WH, Smith L, Churchill ME, Tyler JK.

The C terminus of the histone chaperone Asf1 cross-links to histone H3 in yeast and promotes interaction with histones H3 and H4

Mol Cell Biol. 2013 Feb;33(3):605-21.

Dennehey BK, Noone S, Liu WH, Smith L, Churchill ME, Tyler JK.

PMID: 23184661; PMCID: PMC3666882

Elevated histone expression promotes life span extension

September 10, 2010
Feser J, Truong D, Das C, Carson JJ, Kieft J, Harkness T, Tyler JK.

Elevated histone expression promotes life span extension

Mol Cell. 2010 Sep 10;39(5):724-35.

Feser J, Truong D, Das C, Carson JJ, Kieft J, Harkness T, Tyler JK.

PMID: 20832724; PMCID: PMC3966075

Chaperoning histones during DNA replication and repair

January 22, 2010
Ransom M, Dennehey BK, Tyler JK. Chaperoning histones during DNA replication and repair.

Chaperoning histones during DNA replication and repair

Cell. 2010 Jan 22;140(2):183-95.

Ransom M, Dennehey BK, Tyler JK. 

PMID: 20141833; PMCID: PMC3433953

CBP/p300-mediated acetylation of histone H3 on lysine 56

May 7, 2009
Das C, Lucia MS, Hansen KC, Tyler JK.

CBP/p300-mediated acetylation of histone H3 on lysine 56

Nature. 2009 May 7;459(7243):113-7.

Das C, Lucia MS, Hansen KC, Tyler JK.

Erratum in: Nature. 2009 Aug 27;460(7259):1164.

PMID: 19270680; PMCID: PMC2756583

Acetylation in the globular core of histone H3 on lysine-56 promotes chromatin disassembly during transcriptional activation

July 1, 2008
Williams SK, Truong D, Tyler JK.

Acetylation in the globular core of histone H3 on lysine-56 promotes chromatin disassembly during transcriptional activation

Proc Natl Acad Sci U S A. 2008 Jul 1;105(26):9000-5.

Williams SK, Truong D, Tyler JK.

PMID: 18577595; PMCID: PMC2449354

Structural basis for the histone chaperone activity of Asf1

November 3, 2006
English CM, Adkins MW, Carson JJ, Churchill ME, Tyler JK.

Structural basis for the histone chaperone activity of Asf1

Cell. 2006 Nov 3;127(3):495-508.

English CM, Adkins MW, Carson JJ, Churchill ME, Tyler JK.

PMID: 17081973; PMCID: PMC2981792.

Transcriptional activators are dispensable for transcription in the absence of Spt6-mediated chromatin reassembly of promoter regions

February 3, 2006
Adkins MW, Tyler JK.

Transcriptional activators are dispensable for transcription in the absence of Spt6-mediated chromatin reassembly of promoter regions

Mol Cell. 2006 Feb 3;21(3):405-16.

Adkins MW, Tyler JK.

Erratum in: Mol Cell. 2006 Apr 7;22(1):147-8.

PMID: 16455495

The histone chaperone anti-silencing function 1 is a global regulator of transcription independent of passage through S phase

January 1, 2005
Zabaronick SR, Tyler JK.

The histone chaperone anti-silencing function 1 is a global regulator of transcription independent of passage through S phase

Mol Cell Biol. 2005 Jan;25(2):652-60.

Zabaronick SR, Tyler JK.

Erratum in: Mol Cell Biol. 2005 Apr;25(7):2871.

PMID: 15632066; PMCID: PMC543432

Activation of the DNA damage checkpoint in yeast lacking the histone chaperone anti-silencing function 1

December 1, 2004
Ramey CJ, Howar S, Adkins M, Linger J, Spicer J, Tyler JK.

Activation of the DNA damage checkpoint in yeast lacking the histone chaperone anti-silencing function 1

Mol Cell Biol. 2004 Dec;24(23):10313-27.  

Ramey CJ, Howar S, Adkins M, Linger J, Spicer J, Tyler JK.

PMID: 15542840; PMCID: PMC529054

Chromatin disassembly mediated by the histone chaperone Asf1 is essential for transcriptional activation of the yeast PHO5 and PHO8 genes

June 4, 2004
Adkins MW, Howar SR, Tyler JK.

Chromatin disassembly mediated by the histone chaperone Asf1 is essential for transcriptional activation of the yeast PHO5 and PHO8 genes

Mol Cell. 2004 Jun 4;14(5):657-66.

Adkins MW, Howar SR, Tyler JK.

PMID: 15175160

The RCAF complex mediates chromatin assembly during DNA replication and repair

December 2, 1999
Tyler JK, Adams CR, Chen SR, Kobayashi R, Kamakaka RT, Kadonaga JT.

The RCAF complex mediates chromatin assembly during DNA replication and repair

Nature. 1999 Dec 2;402(6761):555-60.

Tyler JK, Adams CR, Chen SR, Kobayashi R, Kamakaka RT, Kadonaga JT.

PMID: 10591219

The "dark side" of chromatin remodeling: repressive effects on transcription

November 24, 1999
Tyler JK, Kadonaga JT.

The "dark side" of chromatin remodeling: repressive effects on transcription

Cell. 1999 Nov 24;99(5):443-6.

Tyler JK, Kadonaga JT.

PMID: 10589670

Research Faculty

General Contact

Jessica Tyler, Ph.D.
Professor of Pathology and Laboratory Medicine
Weill Cornell Medical College
1300 York Avenue, C4
New York, NY 10065
Tel: 212-746-4092
jet2021@med.cornell.edu

Visit The Tyler Lab Website

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