Title | Multiplexed colorimetric detection of Kaposi's sarcoma associated herpesvirus and Bartonella DNA using gold and silver nanoparticles. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Mancuso M, Jiang L, Cesarman E, Erickson D |
Journal | Nanoscale |
Volume | 5 |
Issue | 4 |
Pagination | 1678-86 |
Date Published | 2013 Feb 21 |
ISSN | 2040-3372 |
Keywords | Bartonella, Colorimetry, DNA, Bacterial, DNA, Viral, Gold, Herpesvirus 8, Human, Metal Nanoparticles, Silver |
Abstract | Kaposi's sarcoma (KS) is an infectious cancer occurring most commonly in human immunodeficiency virus (HIV) positive patients and in endemic regions, such as Sub-Saharan Africa, where KS is among the top four most prevalent cancers. The cause of KS is the Kaposi's sarcoma-associated herpesvirus (KSHV, also called HHV-8), an oncogenic herpesvirus that while routinely diagnosed in developed nations, provides challenges to developing world medical providers and point-of-care detection. A major challenge in the diagnosis of KS is the existence of a number of other diseases with similar clinical presentation and histopathological features, requiring the detection of KSHV in a biopsy sample. In this work we develop an answer to this challenge by creating a multiplexed one-pot detection system for KSHV DNA and DNA from a frequently confounding disease, bacillary angiomatosis. Gold and silver nanoparticle aggregation reactions are tuned for each target and a multi-color change system is developed capable of detecting both targets down to levels between 1 nM and 2 nM. The system developed here could later be integrated with microfluidic sample processing to create a final device capable of solving the two major challenges in point-of-care KS detection. |
DOI | 10.1039/c3nr33492a |
Alternate Journal | Nanoscale |
PubMed ID | 23340972 |
Grant List | UL1 RR 024996 / RR / NCRR NIH HHS / United States |
Related Faculty:
Ethel Cesarman, M.D., Ph.D.